Coding

Part:BBa_K1067006:Design

Designed by: Kristian Davidsen, Helen Cook and Jakob Berg Jespersen   Group: iGEM13_DTU-Denmark   (2013-09-24)

Denitrification operon from Pseudomonas aeruginosa


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3445
    Illegal PstI site found at 6521
    Illegal PstI site found at 7162
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3445
    Illegal PstI site found at 6521
    Illegal PstI site found at 7162
    Illegal NotI site found at 4536
    Illegal NotI site found at 6121
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3445
    Illegal BglII site found at 197
    Illegal BglII site found at 2942
    Illegal BglII site found at 3335
    Illegal XhoI site found at 6639
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3445
    Illegal PstI site found at 6521
    Illegal PstI site found at 7162
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3445
    Illegal PstI site found at 6521
    Illegal PstI site found at 7162
    Illegal NgoMIV site found at 799
    Illegal NgoMIV site found at 1926
    Illegal NgoMIV site found at 1935
    Illegal NgoMIV site found at 2357
    Illegal NgoMIV site found at 2381
    Illegal NgoMIV site found at 2467
    Illegal NgoMIV site found at 3292
    Illegal AgeI site found at 1663
    Illegal AgeI site found at 6506
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 841
    Illegal BsaI.rc site found at 973
    Illegal BsaI.rc site found at 1706
    Illegal BsaI.rc site found at 3391
    Illegal BsaI.rc site found at 3693
    Illegal BsaI.rc site found at 5125
    Illegal BsaI.rc site found at 5676
    Illegal SapI site found at 6182
    Illegal SapI site found at 7434


Design Notes

Genes taken from Pseudomonas aeruginosa PAO1 by colony PCR.

References